areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a s-Dependent Regulator
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چکیده
The areCBA genes in Acinetobacter sp. strain ADP1, determining growth on benzyl alkanoates, are shown to be transcribed as a single operon and regulated by areR, which encodes a regulatory protein of the NtrC/XylR family. Assays of the Are enzymes and of two insertions of lacZ as a reporter gene have shown that the operon is induced by benzyl acetate, benzyl alcohol, and benzaldehyde, as well as 2and 4-hydroxybenzyl acetates and benzyl propionate and butyrate. Two adjacent sites of transcriptional initiation were 97 and 96 bp upstream of the start codon for areC, near a s-dependent 212, 224 promoter. Inactivation of areR and rpoN (for RNA polymerase s) drastically reduced growth rates on the Are substrates and induction of the operon.
منابع مشابه
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